BIT 20% Dosage Guide: Recommended Use Levels & Efficacy Testing
Formulators adding BIT 20% (Benzisothiazolinone, CAS 2634-33-5) to a new paint, adhesive, or metalworking fluid batch need a defensible starting dosage and a way to confirm it works. This guide converts commercial BIT 20% dosage into active ppm, gives application-specific starting ranges drawn from supplier technical data, and explains the ASTM and ISO test methods used to validate the dose before scale-up.
What Dosage of BIT 20% Should I Use?
Most water-based formulations use 0.1%-0.3% BIT 20% commercial product by weight, equivalent to roughly 200-600 ppm active benzisothiazolinone. Metalworking fluid concentrates run higher, at 0.2%-0.5%. The correct dose for your specific formulation must be confirmed with a preservative efficacy test, not assumed from a generic range.
| BIT 20% product dosage | Active BIT (ppm) | Active BIT (% w/w) |
|---|---|---|
| 0.10% | 200 ppm | 0.02% |
| 0.15% | 300 ppm | 0.03% |
| 0.20% | 400 ppm | 0.04% |
| 0.30% | 600 ppm | 0.06% |
| 0.50% | 1,000 ppm | 0.10% |
What Are Typical BIT 20% Use Levels by Application?
Paints, adhesives, and inks typically use 0.1%-0.3% BIT 20%. Metalworking fluid concentrates and detergents needing higher microbial resistance run 0.2%-0.5%. Cosmetic and personal care formulations are dosed at the lower end of the isothiazolinone range and are additionally constrained by regional cosmetic concentration limits.
How Do I Verify a BIT 20% Dose Is Working?
Confirm dosage adequacy with a preservative efficacy (challenge) test: inoculate the finished formulation with defined bacteria and fungi, then measure the reduction in viable organisms over 7-28 days against the acceptance criteria in ASTM D2574 (paints), ASTM E640 (water-containing cosmetics), or ISO 11930 (personal care products).
Frequently asked questions
What is the typical BIT 20% dosage for paint preservation?+
Is BIT 20% dosage based on the active ingredient or the commercial product?+
Which test standard should I use to validate a BIT 20% dose?+
Can I increase the BIT 20% dose if my product fails preservative testing?+
Sources
- ASTM D2574 — Standard Test Method for Resistance of Emulsion Paints in the Container to Attack by Microorganisms (ANSI Webstore)
- ASTM E640 — Standard Test Method for Preservatives in Water-Containing Cosmetics (ANSI Webstore)
- ISO 11930 Preservative Effectiveness Test overview (Microchem Laboratory)
- European Chemicals Agency — Cosmetics preservatives list (CosIng)